Generator

Part:BBa_K2082110:Design

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Weak RBS - dnaQ926 cds - terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 66
    Illegal AgeI site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Intermediate part to make dnaQ926 (BBa_K1333108), dam (BBa_K1500994), seqA, emrR (BBa_K1500995), ugi (BBa_K2082104) and cda1 (BBa_K2082105) ready for expression by cloning downstream of a promoter.


Source

cloning

References